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NEEDHAM INVESTMENT MANAGEMENT LLC
> HQL
| Date | Name | Ticker | Shares | Change | Value | Change | Price |
| 12/31/2022 | TEKLA LIFE SCIENCES INVESTORS | HQL | 299,032 | -150,968 | 4,249,245,000 | 4,243,098,000 | $14,210.00 |
| 09/30/2022 | TEKLA LIFE SCIENCES INVESTORS | HQL | 450,000 | 0 | 6,147,000 | -257,000 | $13.66 |
| 06/30/2022 | TEKLA LIFE SCIENCES INVESTORS | HQL | 450,000 | 0 | 6,404,000 | -1,039,000 | $14.23 |
| 03/31/2022 | TEKLA LIFE SCIENCES INVESTORS | HQL | 450,000 | 0 | 7,443,000 | -1,211,000 | $16.54 |
| 12/31/2021 | TEKLA LIFE SCIENCES INVESTORS | HQL | 450,000 | 0 | 8,654,000 | -994,000 | $19.23 |
| 06/30/2021 | TEKLA LIFE SCIENCES INVESTORS | HQL | 450,000 | -25,000 | 9,648,000 | 285,000 | $21.44 |
| 03/31/2021 | TEKLA LIFE SCIENCES INVESTORS | HQL | 475,000 | 0 | 9,363,000 | -19,000 | $19.71 |
| 12/31/2020 | TEKLA LIFE SCIENCES INVESTORS | HQL | 475,000 | -10,000 | 9,382,000 | 855,000 | $19.75 |
| 09/30/2020 | TEKLA LIFE SCIENCES INVESTORS | HQL | 485,000 | -5,000 | 8,527,000 | -274,000 | $17.58 |
| 06/30/2020 | TEKLA LIFE SCIENCES INVESTORS | HQL | 490,000 | -5,000 | 8,801,000 | 1,386,000 | $17.96 |
| 03/31/2020 | TEKLA LIFE SCIENCES INVESTORS | HQL | 495,000 | -7,359 | 7,415,000 | -1,342,000 | $14.98 |
| 12/31/2019 | TEKLA LIFE SCIENCES INVESTORS | HQL | 502,359 | 0 | 8,757,000 | 1,171,000 | $17.43 |
| 09/30/2019 | TEKLA LIFE SCIENCES INVESTORS | HQL | 502,359 | -17,500 | 7,586,000 | -1,173,000 | $15.10 |
| 06/30/2019 | TEKLA LIFE SCIENCES INVESTORS | HQL | 519,859 | -12,500 | 8,759,000 | -360,000 | $16.85 |
| 03/31/2019 | TEKLA LIFE SCIENCES INVESTORS | HQL | 532,359 | -6,000 | 9,119,000 | 985,000 | $17.13 |
| 12/31/2018 | TEKLA LIFE SCIENCES INVESTORS | HQL | 538,359 | 0 | 8,134,000 | -2,860,000 | $15.11 |
| 09/30/2018 | TEKLA LIFE SCIENCES INVESTORS | HQL | 538,359 | -3,366 | 10,994,000 | 815,000 | $20.42 |
| 06/30/2018 | TEKLA LIFE SCIENCES INVESTORS | HQL | 541,725 | 0 | 10,179,000 | -233,000 | $18.79 |
| 03/31/2018 | TEKLA LIFE SCIENCES INVESTORS | HQL | 541,725 | 2,000 | 10,412,000 | -183,000 | $19.22 |
| 12/31/2017 | TEKLA LIFE SCIENCES INVESTORS | HQL | 539,725 | -10,581 | 10,595,000 | -1,226,000 | $19.63 |
| 09/30/2017 | TEKLA LIFE SCIENCES INVESTORS | HQL | 550,306 | -2,500 | 11,821,000 | 295,000 | $21.48 |
| 06/30/2017 | TEKLA LIFE SCIENCES INVESTORS | HQL | 552,806 | -22,317 | 11,526,000 | 375,000 | $20.85 |
| 03/31/2017 | TEKLA LIFE SCIENCES INVESTORS | HQL | 575,123 | 38,531 | 11,151,000 | 2,034,000 | $19.39 |
| 12/31/2016 | TEKLA LIFE SCIENCES INVESTORS | HQL | 536,592 | 12,257 | 9,117,000 | -704,000 | $16.99 |
| 09/30/2016 | TEKLA LIFE SCIENCES INVESTORS | HQL | 524,335 | 21,403 | 9,821,000 | 1,176,000 | $18.73 |
| 06/30/2016 | TEKLA LIFE SCIENCES INVESTORS | HQL | 502,932 | 502,932 | 8,645,000 | 8,645,000 | $17.19 |
| 03/31/2016 | TEKLA LIFE SCIENCES INVESTORS | HQL | 493,497 | 493,497 | 8,853,000 | 8,853,000 | $17.94 |
| 12/31/2015 | TEKLA LIFE SCIENCES INVESTORS | HQL | 479,143 | 479,143 | 11,327,000 | 11,327,000 | $23.64 |
| 09/30/2015 | TEKLA LIFE SCIENCES INVESTORS | HQL | 470,034 | 470,034 | 10,581,000 | 10,581,000 | $22.51 |
| 06/30/2015 | TEKLA LIFE SCIENCES INVESTORS | HQL | 474,834 | 474,834 | 13,395,000 | 13,395,000 | $28.21 |
| 03/31/2015 | TEKLA LIFE SCIENCES INVESTORS | HQL | 575,034 | 575,034 | 15,854,000 | 15,854,000 | $27.57 |
| 12/31/2014 | TEKLA LIFE SCIENCES INVESTORS | HQL | 622,597 | 622,597 | 15,490,000 | 15,490,000 | $24.88 |
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