The Securities and Exchange Commission has not necessarily reviewed the information in this filing and has not determined if it is accurate and complete.
The reader should not assume that the information is accurate and complete.

UNITED STATES SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549
FORM 13F

FORM 13F INFORMATION TABLE
OMB APPROVAL
OMB Number: 3235-0006
Estimated average burden
hours per response: 23.8
COLUMN 1 COLUMN 2 COLUMN 3 COLUMN 4 COLUMN 5 COLUMN 6 COLUMN 7 COLUMN 8
VALUE SHRS OR SH/ PUT/ INVESTMENT OTHER VOTING AUTHORITY
NAME OF ISSUER TITLE OF CLASS CUSIP (x$1000) PRN AMT PRN CALL DISCRETION MANAGER SOLE SHARED NONE
1LIFE HEALTHCARE INC COM 68269G107 28,798 1,586,652 SH   DFND   0 0 1,586,652
2U INC COM 90214J101 687 32,379 SH   DFND   0 0 32,379
ABIOMED INC COM 003654100 19,993 137,731 SH   DFND   0 0 137,731
ACACIA COMMUNICATIONS INC COM 00401C108 575 8,565 SH   DFND   0 0 8,565
ACCEL ENTERTAINMENT INC COM CL A1 00436Q106 18 2,400 SH   DFND   0 0 2,400
ACTIVISION BLIZZARD INC COM 00507V109 1,210 20,342 SH   DFND   0 0 20,342
ADAPTIVE BIOTECHNOLOGIES COR COM 00650F109 63,318 2,279,250 SH   DFND   0 0 2,279,250
ADESTO TECHNOLOGIES CORP COM 00687D101 10,987 981,830 SH   DFND   0 0 981,830
ADOBE INC COM 00724F101 45,868 144,130 SH   DFND   0 0 144,130
ADYEN NV 144A COM N3501V104 29,293 34,644 SH   DFND   0 0 34,644
AEROJET ROCKETDYNE HLDGS INC COM 007800105 40,853 976,651 SH   DFND   0 0 976,651
AFYA LTD CL A COM G01125106 6,455 338,662 SH   DFND   0 0 338,662
ALASKA COMMUNICATIONS SYS GR COM 01167P101 1,927 1,107,622 SH   DFND   0 0 1,107,622
ALECTOR INC COM 014442107 65,788 2,726,389 SH   DFND   0 0 2,726,389
ALIBABA GROUP HLDG LTD SPONSORED ADS 01609W102 75,018 385,734 SH   DFND   0 0 385,734
ALLAKOS INC COM 01671P100 3,371 75,759 SH   DFND   0 0 75,759
ALLEGIANT TRAVEL CO COM 01748X102 7,240 88,509 SH   DFND   0 0 88,509
ALNYLAM PHARMACEUTICALS INC COM 02043Q107 100,052 919,177 SH   DFND   0 0 919,177
ALPHABET INC CAP STK CL A 02079K305 50,156 43,165 SH   DFND   0 0 43,165
ALPHABET INC CAP STK CL C 02079K107 55,816 48,001 SH   DFND   0 0 48,001
ALTERYX INC COM CL A 02156B103 3,431 36,047 SH   DFND   0 0 36,047
ALTUS MIDSTREAM CO CL A 02215L100 1,193 1,591,184 SH   DFND   0 0 1,591,184
AMAZON COM INC COM 023135106 343,261 176,057 SH   DFND   0 0 176,057
AMERICAN TOWER CORP NEW COM 03027X100 17,253 79,231 SH   DFND   0 0 79,231
AMERICOLD RLTY TR COM 03064D108 1,369 40,228 SH   DFND   0 0 40,228
AMYRIS INC COM NEW 03236M200 130 50,718 SH   DFND   0 0 50,718
ANAPLAN INC COM 03272L108 5,614 185,531 SH   DFND   0 0 185,531
ANTERIX INC COM 03676C100 272 5,953 SH   DFND   0 0 5,953
APACHE CORP COM 037411105 1,062 254,101 SH   DFND   0 0 254,101
APPFOLIO INC COM CL A 03783C100 53,067 478,301 SH   DFND   0 0 478,301
APPIAN CORP CL A 03782L101 1,641 40,787 SH   DFND   0 0 40,787
APPLE INC COM 037833100 6,971 27,415 SH   DFND   0 0 27,415
APPLIED OPTOELECTRONICS INC COM 03823U102 54 7,050 SH   DFND   0 0 7,050
APTOSE BIOSCIENCES INC COM NEW 03835T200 1 125 SH   DFND   0 0 125
ARCO PLATFORM LTD COM CL A G04553106 12 285 SH   DFND   0 0 285
ARCUS BIOSCIENCES INC COM 03969F109 279 20,130 SH   DFND   0 0 20,130
ARGENX SE SPONSORED ADR 04016X101 819 6,215 SH   DFND   0 0 6,215
ARISTA NETWORKS INC COM 040413106 81,504 402,388 SH   DFND   0 0 402,388
ARITZIA INC SUB VTG SHS ISIN CA04045U1021 SEDOL BDCG2C2 COM 04045U102 11,834 1,368,259 SH   DFND   0 0 1,368,259
ARVINAS INC COM 04335A105 63,524 1,576,272 SH   DFND   0 0 1,576,272
ATLASSIAN CORP PLC CL A G06242104 26,945 196,305 SH   DFND   0 0 196,305
AUTO TRADER GROUP PLC ORD GBP001 ISIN GB00BVYVFW23 SEDOL BVYV COM G06708104 1,785 327,783 SH   DFND   0 0 327,783
AUTODESK INC COM 052769106 92,984 595,670 SH   DFND   0 0 595,670
AXIS BANK GDR EACH REPR 1 INR10 REG S ISIN US05462W1099 SEDOL COM 05462W109 382 15,085 SH   DFND   0 0 15,085
AXOGEN INC COM 05463X106 280 26,940 SH   DFND   0 0 26,940
AZUL S A SPONSR ADR PFD 05501U106 33,802 3,317,193 SH   DFND   0 0 3,317,193
BASICFIT NV 144A REG S ISIN NL0011872650 SEDOL BD9Y9B7 COM N10058100 7 427 SH   DFND   0 0 427
BEAM THERAPEUTICS INC COM 07373V105 1,617 89,813 SH   DFND   0 0 89,813
BENEFYTT TECHNOLOGIES INC COM CL A 08182C106 291 13,010 SH   DFND   0 0 13,010
BERKLEY W R CORP COM 084423102 4,448 85,261 SH   DFND   0 0 85,261
BERKSHIRE HATHAWAY INC DEL CL B NEW 084670702 501 2,739 SH   DFND   0 0 2,739
BEYOND MEAT INC COM 08862E109 26,333 395,388 SH   DFND   0 0 395,388
BIOHAVEN PHARMACTL HLDG CO L COM G11196105 3,476 102,159 SH   DFND   0 0 102,159
BK OF AMERICA CORP COM 060505104 1 35 SH   DFND   0 0 35
BLUEBIRD BIO INC COM 09609G100 25,432 553,361 SH   DFND   0 0 553,361
BOSTON SCIENTIFIC CORP COM 101137107 751 23,029 SH   DFND   0 0 23,029
CALIX INC COM 13100M509 11,252 1,589,330 SH   DFND   0 0 1,589,330
CARL ZEISS MEDITEC AG NPV ISIN DE0005313704 SEDOL 5922961 COM D14895102 1,186 12,231 SH   DFND   0 0 12,231
CARVANA CO CL A 146869102 48,306 876,850 SH   DFND   0 0 876,850
CASPER SLEEP INC COM 147626105 52 12,047 SH   DFND   0 0 12,047
CATAPULT GROUP INTERNATIONAL ISIN AU000000CAT9 SEDOL BTL0Y76 COM Q2159P101 86 186,848 SH   DFND   0 0 186,848
CELLNEX TELECOM SAU EUR025 144A REG S ISIN ES0105066007 SEDOL COM E2R41M104 5,711 125,577 SH   DFND   0 0 125,577
CHART INDS INC COM PAR $0.01 16115Q308 25 860 SH   DFND   0 0 860
CHEGG INC COM 163092109 60,022 1,677,542 SH   DFND   0 0 1,677,542
CHEWY INC CL A 16679L109 448 11,937 SH   DFND   0 0 11,937
CHIPOTLE MEXICAN GRILL INC COM 169656105 1 1 SH   DFND   0 0 1
CITIGROUP INC COM NEW 172967424 1 35 SH   DFND   0 0 35
CLOUDFLARE INC CL A COM 18915M107 86,198 3,671,111 SH   DFND   0 0 3,671,111
CODEXIS INC COM 192005106 30,787 2,758,707 SH   DFND   0 0 2,758,707
COLLATERAL DELV TO BANK OF AMERICA NA SECURITIES ON LOAN NOT COV COM L0C990089 0 907,358 SH   DFND   0 0 907,358
CONTROLADORA VUELA CIA DE AV SPON ADR RP 10 21240E105 9,596 2,797,775 SH   DFND   0 0 2,797,775
CORECIVIC INC COM 21871N101 90 8,079 SH   DFND   0 0 8,079
COUPA SOFTWARE INC COM 22266L106 20,967 150,057 SH   DFND   0 0 150,057
COVANTA HLDG CORP COM 22282E102 5,952 696,141 SH   DFND   0 0 696,141
COVETRUS INC COM 22304C100 0 8 SH   DFND   0 0 8
CRIMSON WINE GROUP COM USD001 COM 22662X100 530 90,678 SH   DFND   0 0 90,678
CROWDSTRIKE HLDGS INC CL A 22788C105 13,991 251,269 SH   DFND   0 0 251,269
DATADOG INC CL A COM 23804L103 15,960 443,591 SH   DFND   0 0 443,591
DENALI THERAPEUTICS INC COM 24823R105 57,100 3,261,022 SH   DFND   0 0 3,261,022
DEXCOM INC COM 252131107 158,545 588,796 SH   DFND   0 0 588,796
DIAMOND EAGLE ACQUISITION CO COM CL A 25258L109 13 1,080 SH   DFND   0 0 1,080
DIAMONDBACK ENERGY INC COM 25278X109 549 20,959 SH   DFND   0 0 20,959
DIGI INTL INC COM 253798102 623 65,332 SH   DFND   0 0 65,332
DINE BRANDS GLOBAL INC COM 254423106 667 23,270 SH   DFND   0 0 23,270
DISH NETWORK CORPORATION CL A 25470M109 25,648 1,283,036 SH   DFND   0 0 1,283,036
DISNEY WALT CO COM DISNEY 254687106 3,459 35,808 SH   DFND   0 0 35,808
DOCUSIGN INC COM 256163106 135,159 1,462,756 SH   DFND   0 0 1,462,756
DOWNEY FINL CORP COM 261018105 0 200 SH   DFND   0 0 200
EHEALTH INC COM 28238P109 36,844 261,636 SH   DFND   0 0 261,636
EIDOS THERAPEUTICS INC COM 28249H104 17,540 358,034 SH   DFND   0 0 358,034
ELASTIC N V ORD SHS N14506104 2,804 50,249 SH   DFND   0 0 50,249
ENPHASE ENERGY INC COM 29355A107 86,608 2,682,194 SH   DFND   0 0 2,682,194
ENTERCOM COMMUNICATIONS CORP CL A 293639100 314 183,734 SH   DFND   0 0 183,734
EPAM SYS INC COM 29414B104 72,717 391,668 SH   DFND   0 0 391,668
EQUATOR EXPLORATION COM STK NPV COM G3084F128 0 215,000 SH   DFND   0 0 215,000
EROS INTL PLC SHS NEW G3788M114 68 41,348 SH   DFND   0 0 41,348
ESPERION THERAPEUTICS INC NE COM 29664W105 36 1,128 SH   DFND   0 0 1,128
ETSY INC COM 29786A106 19,741 513,549 SH   DFND   0 0 513,549
EXACT SCIENCES CORP COM 30063P105 90,598 1,562,036 SH   DFND   0 0 1,562,036
FACEBOOK INC CL A 30303M102 62,704 375,921 SH   DFND   0 0 375,921
FASTLY INC CL A 31188V100 11,498 605,815 SH   DFND   0 0 605,815
FERGUSON PLC ORD GBP010 ISIN JE00BJVNSS43 SEDOL BJVNSS4 COM G3421J106 8,131 129,693 SH   DFND   0 0 129,693
FIVE BELOW INC COM 33829M101 43,978 624,869 SH   DFND   0 0 624,869
FLEX LNG LTD BM USD010 ISIN BMG359472021 SEDOL BJJMGB2 COM ZZCKKNHC2 675 133,455 SH   DFND   0 0 133,455
FLEXION THERAPEUTICS INC COM 33938J106 219 27,832 SH   DFND   0 0 27,832
FLOOR & DECOR HLDGS INC CL A 339750101 3,391 105,681 SH   DFND   0 0 105,681
FORESCOUT TECHNOLOGIES INC COM 34553D101 1,293 40,927 SH   DFND   0 0 40,927
FOUR SEAS MERCANTILE HLDGS HKD010 ISIN KYG365451049 SEDOL 634 COM G36545104 101 270,000 SH   DFND   0 0 270,000
FRANCO NEVADA CORP COM 351858105 7,326 73,616 SH   DFND   0 0 73,616
FRESHPET INC COM 358039105 144,687 2,265,343 SH   DFND   0 0 2,265,343
FRIENDLY ENERGY EXPLORATION COM NPV COM 35846A200 0 690 SH   DFND   0 0 690
GALAPAGOS NV NPV ISIN BE0003818359 SEDOL B07Q2V5 COM B44170106 129,714 653,136 SH   DFND   0 0 653,136
GASLOG LTD SHS G37585109 37 10,265 SH   DFND   0 0 10,265
GEOPARK LTD USD SHS G38327105 2,994 423,481 SH   DFND   0 0 423,481
GILEAD SCIENCES INC COM 375558103 2,073 27,729 SH   DFND   0 0 27,729
GLAUKOS CORP COM 377322102 28,836 934,413 SH   DFND   0 0 934,413
GLOBAL BLOOD THERAPEUTICS IN COM 37890U108 1 13 SH   DFND   0 0 13
GLOBANT S A COM L44385109 63,801 725,996 SH   DFND   0 0 725,996
GOLAR LNG LTD SHS G9456A100 74 9,393 SH   DFND   0 0 9,393
GOLDEN MINERALS CO COM 381119106 10 47,017 SH   DFND   0 0 47,017
GOLDMAN SACHS GROUP INC COM 38141G104 64,080 414,517 SH   DFND   0 0 414,517
GRID DYNAMICS HLDGS INC COM CL A 39813G109 202 25,246 SH   DFND   0 0 25,246
GROCERY OUTLET HLDG CORP COM 39874R101 3,421 99,627 SH   DFND   0 0 99,627
GTT COMMUNICATIONS INC COM 362393100 10,132 1,274,521 SH   DFND   0 0 1,274,521
GUARDANT HEALTH INC COM 40131M109 714 10,253 SH   DFND   0 0 10,253
HDFC BANK LTD SPONSORED ADS 40415F101 5,616 146,034 SH   DFND   0 0 146,034
HEALTHEQUITY INC COM 42226A107 3,308 65,393 SH   DFND   0 0 65,393
HEARUSA INC NEW COM 422360305 0 375 SH   DFND   0 0 375
HEICO CORP NEW CL A 422806208 3,757 58,795 SH   DFND   0 0 58,795
HELLOFRESH SE SHS ISIN DE000A161408 SEDOL BYWH8S0 COM D3R2MA100 54,262 1,616,098 SH   DFND   0 0 1,616,098
HOWARD HUGHES CORP COM 44267D107 186 3,686 SH   DFND   0 0 3,686
HUBSPOT INC COM 443573100 89,180 669,571 SH   DFND   0 0 669,571
ICICI BANK LIMITED ADR 45104G104 3,011 354,195 SH   DFND   0 0 354,195
ILLUMINA INC COM 452327109 4,863 17,807 SH   DFND   0 0 17,807
IMPINJ INC COM 453204109 798 47,732 SH   DFND   0 0 47,732
INPHI CORP COM 45772F107 710 8,967 SH   DFND   0 0 8,967
INSPIRE MED SYS INC COM 457730109 56,173 931,871 SH   DFND   0 0 931,871
INSULET CORP COM 45784P101 3,031 18,296 SH   DFND   0 0 18,296
INTERBREW PLC GDR EACH REPR 1 A SHS SPON REG SEUR COM 45845T308 0 200 SH   DFND   0 0 200
INTL GNRL INSURANCE HLDNGS L SHS G4809J106 17,492 2,186,440 SH   DFND   0 0 2,186,440
INTUITIVE SURGICAL INC COM NEW 46120E602 92,241 186,266 SH   DFND   0 0 186,266
INVITAE CORP COM 46185L103 86,261 6,310,263 SH   DFND   0 0 6,310,263
IRHYTHM TECHNOLOGIES INC COM 450056106 28,300 347,876 SH   DFND   0 0 347,876
JPMORGAN CHASE & CO COM 46625H100 1,528 16,974 SH   DFND   0 0 16,974
KAMBI GROUP PLC EUR0003 ISIN MT0000780107 SEDOL BMNQDC1 COM X4170A107 10 1,097 SH   DFND   0 0 1,097
KB HOME COM 48666K109 153 8,437 SH   DFND   0 0 8,437
KIRKLAND LAKE GOLD LTD COM 49741E100 11,330 382,767 SH   DFND   0 0 382,767
KORNIT DIGITAL LTD SHS M6372Q113 24,206 972,505 SH   DFND   0 0 972,505
KRYSTAL BIOTECH INC COM 501147102 2 37 SH   DFND   0 0 37
KURA SUSHI USA INC CL A COM 501270102 638 53,377 SH   DFND   0 0 53,377
LGI HOMES INC COM 50187T106 58,692 1,299,944 SH   DFND   0 0 1,299,944
LHC GROUP INC COM 50187A107 2,354 16,787 SH   DFND   0 0 16,787
LI NING CO LTD HKD01 ISIN KYG5496K1242 SEDOL B01JCK9 COM G5496K124 13 4,391 SH   DFND   0 0 4,391
LIBERTY MEDIA CORP DEL COM A BRAVES GRP 531229706 2,788 142,956 SH   DFND   0 0 142,956
LIBERTY MEDIA CORP DEL COM C BRAVES GRP 531229888 7,482 392,547 SH   DFND   0 0 392,547
LIBERTY MEDIA CORP DEL COM SER C FRMLA 531229854 1,032 37,901 SH   DFND   0 0 37,901
LIVE NATION ENTERTAINMENT IN COM 538034109 303 6,655 SH   DFND   0 0 6,655
LIVEPERSON INC COM 538146101 26,035 1,144,399 SH   DFND   0 0 1,144,399
LIVONGO HEALTH INC COM 539183103 25,476 892,968 SH   DFND   0 0 892,968
LOVESAC COMPANY COM 54738L109 166 28,461 SH   DFND   0 0 28,461
LUCKIN COFFEE INC SPONSORED ADS 54951L109 14,414 530,131 SH   DFND   0 0 530,131
LULULEMON ATHLETICA INC COM 550021109 6,539 34,497 SH   DFND   0 0 34,497
LUMENTUM HLDGS INC COM 55024U109 732 9,931 SH   DFND   0 0 9,931
LYFT INC CL A COM 55087P104 27,225 1,013,957 SH   DFND   0 0 1,013,957
MAG SILVER CORP COM 55903Q104 10,775 1,402,981 SH   DFND   0 0 1,402,981
MAGENTA THERAPEUTICS INC COM 55910K108 25,495 4,059,789 SH   DFND   0 0 4,059,789
MAGNOLIA OIL & GAS CORP CL A 559663109 2,574 643,556 SH   DFND   0 0 643,556
MARTIN MARIETTA MATLS INC COM 573284106 71,395 377,294 SH   DFND   0 0 377,294
MASTERCARD INC CL A 57636Q104 20,344 84,219 SH   DFND   0 0 84,219
MATCH GROUP INC COM 57665R106 62,064 939,788 SH   DFND   0 0 939,788
MAYVILLE ENGINEERING CO INC COM 578605107 757 123,482 SH   DFND   0 0 123,482
MERCADOLIBRE INC COM 58733R102 75,565 154,663 SH   DFND   0 0 154,663
MICRON TECHNOLOGY INC COM 595112103 638 15,166 SH   DFND   0 0 15,166
MICROSOFT CORP COM 594918104 42,572 269,937 SH   DFND   0 0 269,937
MOLECULAR TEMPLATES INC COM 608550109 9,577 720,582 SH   DFND   0 0 720,582
MONGODB INC CL A 60937P106 124,416 911,205 SH   DFND   0 0 911,205
MONOLITHIC PWR SYS INC COM 609839105 15,756 94,087 SH   DFND   0 0 94,087
NATERA INC COM 632307104 34,114 1,142,453 SH   DFND   0 0 1,142,453
NATIONAL VISION HLDGS INC COM 63845R107 11 587 SH   DFND   0 0 587
NEOPHOTONICS CORP COM 64051T100 949 130,913 SH   DFND   0 0 130,913
NETFLIX INC COM 64110L106 473,221 1,260,242 SH   DFND   0 0 1,260,242
NEW PACIFIC METALS CORP COM NPV ISIN CA6478241012 SEDOL BF4CP8 COM 647824101 4,912 1,412,324 SH   DFND   0 0 1,412,324
NEW YORK TIMES CO CL A 650111107 92,148 3,000,594 SH   DFND   0 0 3,000,594
NIKE INC CL B 654106103 9,851 119,059 SH   DFND   0 0 119,059
NOBLE MIDSTREAM PARTNERS LP COM UNIT REPST 65506L105 3 947 SH   DFND   0 0 947
NV5 GLOBAL INC COM 62945V109 27 644 SH   DFND   0 0 644
NVIDIA CORP COM 67066G104 46,670 177,047 SH   DFND   0 0 177,047
OCADO GROUP PLC ORD GBP002 ISIN GB00B3MBS747 SEDOL B3MBS74 COM G6718L106 175,387 11,594,000 SH   DFND   0 0 11,594,000
OKTA INC CL A 679295105 210,596 1,722,526 SH   DFND   0 0 1,722,526
ONEWATER MARINE INC CL A COM 68280L101 4,864 635,823 SH   DFND   0 0 635,823
OPTION CARE HEALTH INC COM NEW 68404L201 35,553 3,754,279 SH   DFND   0 0 3,754,279
PAGERDUTY INC COM 69553P100 895 51,783 SH   DFND   0 0 51,783
PALOMAR HLDGS INC COM 69753M105 11,909 204,761 SH   DFND   0 0 204,761
PARAMOUNT RESOURCES LIMITED COM NPV CLASS A ISIN CA6993202069 S COM 699320206 118 148,418 SH   DFND   0 0 148,418
PAYPAL HLDGS INC COM 70450Y103 10,525 109,935 SH   DFND   0 0 109,935
PDF SOLUTIONS INC COM 693282105 322 27,448 SH   DFND   0 0 27,448
PELOTON INTERACTIVE INC CL A COM 70614W100 86,897 3,272,970 SH   DFND   0 0 3,272,970
PENN NATL GAMING INC COM 707569109 342 27,075 SH   DFND   0 0 27,075
PENN TREATY AMERN CORP COM NEW COM 707874400 0 125 SH   DFND   0 0 125
PENSKE AUTOMOTIVE GRP INC COM 70959W103 1,855 66,253 SH   DFND   0 0 66,253
PENUMBRA INC COM 70975L107 512 3,175 SH   DFND   0 0 3,175
PINDUODUO INC SPONSORED ADS 722304102 583 16,188 SH   DFND   0 0 16,188
PINTEREST INC CL A 72352L106 208 13,482 SH   DFND   0 0 13,482
POOL CORPORATION COM 73278L105 69,954 355,510 SH   DFND   0 0 355,510
PREVAIL THERAPEUTICS INC COM 74140Y101 0 5 SH   DFND   0 0 5
PROLOGIS INC. COM 74340W103 1,209 15,042 SH   DFND   0 0 15,042
PROOFPOINT INC COM 743424103 4,759 46,391 SH   DFND   0 0 46,391
PURPLE INNOVATION INC COM 74640Y106 5,519 971,699 SH   DFND   0 0 971,699
Q2 HLDGS INC COM 74736L109 57,863 979,726 SH   DFND   0 0 979,726
QUANTERIX CORP COM 74766Q101 57,523 3,131,375 SH   DFND   0 0 3,131,375
QUIDEL CORP COM 74838J101 3,777 38,616 SH   DFND   0 0 38,616
QUOTIENT LTD SHS G73268107 2,199 556,814 SH   DFND   0 0 556,814
REDFIN CORP COM 75737F108 1,203 78,033 SH   DFND   0 0 78,033
REECE LIMITED NPV ISIN AU000000REH4 SEDOL 6728801 COM Q80528138 3,328 597,471 SH   DFND   0 0 597,471
REGENERON PHARMACEUTICALS COM 75886F107 3,484 7,135 SH   DFND   0 0 7,135
RENALYTIX AI PLC ORD GBP00025 REG S REG S ISIN GB00BYWL4Y04 COM G7489R108 534 195,754 SH   DFND   0 0 195,754
REPLIGEN CORP COM 759916109 49,647 514,266 SH   DFND   0 0 514,266
RIGHTMOVE ORD GBP0001 ISIN GB00BGDT3G23 SEDOL BGDT3G2 COM G7565D106 5,657 935,978 SH   DFND   0 0 935,978
RINGCENTRAL INC CL A 76680R206 245,160 1,156,908 SH   DFND   0 0 1,156,908
ROKU INC COM CL A 77543R102 240,316 2,747,097 SH   DFND   0 0 2,747,097
SAFARICOM PLC KES005 ISIN KE1000001402 SEDOL B2QN3J6 COM V74587102 26,749 106,238,516 SH   DFND   0 0 106,238,516
SAFETY INS GROUP INC COM 78648T100 392 4,645 SH   DFND   0 0 4,645
SALESFORCE COM INC COM 79466L302 75,757 526,166 SH   DFND   0 0 526,166
SAREPTA THERAPEUTICS INC COM 803607100 90,805 928,282 SH   DFND   0 0 928,282
SCORPIO TANKERS INC SHS Y7542C130 261 13,632 SH   DFND   0 0 13,632
SEMLER SCIENTIFIC INC COM USD0001 COM 81684M104 3,758 94,184 SH   DFND   0 0 94,184
SENESTECH INC COM NEW 81720R208 90 43,701 SH   DFND   0 0 43,701
SENSEONICS HLDGS INC COM 81727U105 9,806 15,478,543 SH   DFND   0 0 15,478,543
SERES THERAPEUTICS INC COM 81750R102 372 104,269 SH   DFND   0 0 104,269
SERVICENOW INC COM 81762P102 29,363 102,461 SH   DFND   0 0 102,461
SHAKE SHACK INC CL A 819047101 83,129 2,202,678 SH   DFND   0 0 2,202,678
SHERWIN WILLIAMS CO COM 824348106 863 1,879 SH   DFND   0 0 1,879
SHOCKWAVE MED INC COM 82489T104 47,286 1,425,146 SH   DFND   0 0 1,425,146
SHOPIFY INC CL A 82509L107 194,034 465,388 SH   DFND   0 0 465,388
SHOTSPOTTER INC COM 82536T107 57,427 2,089,776 SH   DFND   0 0 2,089,776
SI BONE INC COM 825704109 15,623 1,307,330 SH   DFND   0 0 1,307,330
SILK RD MED INC COM 82710M100 2,532 80,432 SH   DFND   0 0 80,432
SILVERCREST METALS INC COM 828363101 50,252 9,626,887 SH   DFND   0 0 9,626,887
SINTX TECHNOLOGIES INC COM NEW 829392307 0 71 SH   DFND   0 0 71
SITEONE LANDSCAPE SUPPLY INC COM 82982L103 20,531 278,883 SH   DFND   0 0 278,883
SITIME CORP COM 82982T106 864 39,671 SH   DFND   0 0 39,671
SLACK TECHNOLOGIES INC COM CL A 83088V102 57,831 2,154,657 SH   DFND   0 0 2,154,657
SMARTSHEET INC COM CL A 83200N103 236 5,681 SH   DFND   0 0 5,681
SMILEDIRECTCLUB INC CL A COM 83192H106 6 1,300 SH   DFND   0 0 1,300
SNAP INC CL A 83304A106 37,478 3,152,061 SH   DFND   0 0 3,152,061
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SPLUNK INC COM 848637104 5,977 47,349 SH   DFND   0 0 47,349
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STAAR SURGICAL CO COM PAR $0.01 852312305 7 206 SH   DFND   0 0 206
STAMPS COM INC COM NEW 852857200 232 1,784 SH   DFND   0 0 1,784
STAR BULK CARRIERS CORP. SHS PAR Y8162K204 108 19,226 SH   DFND   0 0 19,226
STITCH FIX INC COM CL A 860897107 48 3,754 SH   DFND   0 0 3,754
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STONECO LTD COM CL A G85158106 26,423 1,213,720 SH   DFND   0 0 1,213,720
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T MOBILE US INC COM 872590104 74,492 887,868 SH   DFND   0 0 887,868
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TANDEM DIABETES CARE INC COM NEW 875372203 81,515 1,266,746 SH   DFND   0 0 1,266,746
TELADOC HEALTH INC COM 87918A105 164,021 1,058,131 SH   DFND   0 0 1,058,131
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TESLA INC COM 88160R101 283,356 540,757 SH   DFND   0 0 540,757
THE A2 MILK COMPANY LTD NPV ISIN NZATME0002S8 SEDOL BWSRTS7 COM ZZCXTSUT2 2,015 198,288 SH   DFND   0 0 198,288
THOR INDS INC COM 885160101 2,545 60,332 SH   DFND   0 0 60,332
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TWILIO INC CL A 90138F102 3,579 39,992 SH   DFND   0 0 39,992
TWIST BIOSCIENCE CORP COM 90184D100 433 14,169 SH   DFND   0 0 14,169
TWITTER INC COM 90184L102 1,930 78,563 SH   DFND   0 0 78,563
UBER TECHNOLOGIES INC COM 90353T100 1,141 40,857 SH   DFND   0 0 40,857
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VEEVA SYS INC CL A COM 922475108 9,762 62,432 SH   DFND   0 0 62,432
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VISA INC COM CL A 92826C839 343 2,131 SH   DFND   0 0 2,131
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WAYFAIR INC CL A 94419L101 608 11,382 SH   DFND   0 0 11,382
WHEATON PRECIOUS METALS CORP COM 962879102 2,190 79,555 SH   DFND   0 0 79,555
WILLSCOT CORP COM 971375126 203 20,000 SH   DFND   0 0 20,000
WORKDAY INC CL A 98138H101 17,374 133,424 SH   DFND   0 0 133,424
WUXI BIOLOGICS CAYMAN INC USD0000025 144A REG S ISIN KYG9700 COM G97008109 28,340 2,185,656 SH   DFND   0 0 2,185,656
XP INC CL A G98239109 8,142 422,104 SH   DFND   0 0 422,104
ZAI LAB LTD ADR 98887Q104 59,617 1,158,066 SH   DFND   0 0 1,158,066
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ZILLOW GROUP INC CL C CAP STK 98954M200 4,733 131,393 SH   DFND   0 0 131,393
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