<DOCUMENT>
<TYPE>SC 13D/A
<SEQUENCE>1
<FILENAME>nsph0712.txt
<DESCRIPTION>NANOSPHERE 13D/A
<TEXT>
			     UNITED STATES
		  SECURITIES AND EXCHANGE COMMISSION
			Washington, D.C.  20549

			      SCHEDULE 13D

		Under the Securities Exchange Act of 1934
			   (Amendment No. 2)


			      Nanosphere, Inc
			     (Name of Issuer)


				Common
		    (Title of Class of Securities)


			        63009F105
			     (CUSIP Number)


Richard C. Perkins, 730 Lake St E, Wayzata, MN 55391, 952-473-8367
    (Name, Address and Telephone Number of Person Authorized)


			    6/30/2016
 	   (Date of Event which Requires Filing of this Statement)


If the filing person has previously filed a statement on Schedule 13G to
report the acquisition that is the subject of this Schedule 13D, and is
filing this schedule because of ss240.13d-1(e), 240.13d-1(f) or 240.13d-1(g),
check the following box. / /

Note: Schedules filed in paper format shall include a signed original and
five copies of the schedule, including all exhibits.  See s240.13d-7 for
other parties to whom copies are to be sent.

The remainder of this cover page shall be filled out for a reporting person's
initial filing on this form with respect to the subject class of securities,
and for any subsequent amendment containing information which
would alter disclosures provided in a prior cover page.

The information required in the remainder of this cover page shall not be
deemed to be "filed" for the purpose of Section 18 of the Securities
Exchange Act of 1934 ("Act") or otherwise subject to the liabilities of
that section of the Act but shall be subject to all other provisions of
the Act (however, see the Notes).

Potential persons who are to respond to the collection of information
contained in this form are not required to respond unless the form
displays a currently valid OMB control number.


CUSIP No.  63009F105


1.Names of Reporting Persons.
I.R.S. Identification Nos. of above persons (entities only).

Perkins Capital Management, Inc.
41-1501962

2.Check the Appropriate Box if a Member of a Group (See Instructions)

(a) /  /

(b) /  /

3.SEC Use Only


4.Source of Funds (See Instructions)

OO


6.Citizenship or Place of Organization

730 East Lake Street, Wayzata, Minnesota  55391

Number of	7.Sole Voting Power
		143,000
Shares
Beneficially	8.Shared Voting Power
owned		0

by Each		9.Sole Dispositive Power
		143,000
Reporting
Person		10.Shared Dispositive Power With
		0


11.	Aggregate Amount Beneficially Owned by Each Reporting Person

	143,000

12.	Check if the Aggregate Amount in Row (11) Excludes Certain Shares


13.	Percent of Class Represented by Amount in Row (11)

	1.1%

14.	Type of Reporting Person (See Instructions)

	IA


GENERAL INSTRUCTIONS

Item 1.  Security and Issuer:

Nanosphere, Inc
4088 Commercial Avenue
Northbrook, IL  60062

Item 2.  Identity and Background:

(a)Name:

Perkins Capital Management, Inc.

(b)Business Address:

730 Lake St E, Wayzata, MN  55391

c)Present Principal occupation or employment and the name,
principal business and address of any corporation or other organization
in which such employment is conducted;

Perkins Capital Management, Inc. is a federally registered investment
adviser.

(d) Whether or not, during the last five years, such person has been
convicted in a criminal proceeding (excluding traffic violation or similar
misdemeanors) and, if so, give the dates, nature of conviction, name and
location of court, and penalty imposed, or other disposition of the case;

No named person has been convicted in a criminal proceeding

(e) Whether or not, during the last five years, such person was a party
to a civil proceeding of a judicial or administrative body of competent
jurisdiction and as a result of such proceeding was or is subject to a
judgment, decree or final order enjoining future violations of, or
prohibiting or mandating activities subject to, federal or state
securities laws or finding any violation with respect to such laws;
and, if so, identify and describe such proceedings and summarize
the terms of such judgement, decree or final order; and

No named person has been party to such incidents

(f)Citizenship

Perkins Capital Managment, Inc. is a Minnesota Corporation

Item 3.  Source and Amount of Funds or Other Consideration

The funds used in purchasing shares on behalf of clients of
Perkins Capital Management, Inc. come from various client accounts
in which Perkins Capital Management, Inc. disclaims beneficial
ownership.


Item 4.  Purpose of Transaction

State the purpose or purposes of the acquisition of securities of the issuer.
Describe any plans or proposals which the reporting persons may have
which relate to or would result in:

Perkins Capital Management, Inc. transactions are for client accounts and
in the normal course of business.

Item 5.  Interest in Securities of the Issuer

Perkins Capital Management, Inc. holds 143,000 common equivalents and
0 warrants exerciseable within 60 days for clients and has sole
voting power over 143,000 of these shares and sole dispositive power
over 143,000.  Perkins Capital Management, Inc. disclaims
beneficial interest in all shares held in client accounts.

Item 6.  Contracts, Arrangements, Understandings or Relationships with
 Respect to Securities of the Issuer

Perkins Capital Managment, Inc. is a federally registered investment
adviser and has no contracts, arragements, understandings or other
relationship with respect to the securities of the issuer.

Item 7.  Material to Be Filed as Exhibits:

None

Signature

After reasonable inquiry and to the best of my knowledge and belief, I certify
that the information set forth in this statement is true, complete and correct.


Date:  July 12, 2016


/s/Richard C. Perkins

Signature

Richard C. Perkins/Executive Vice President
Name/Title
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